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Thesis/Dissertation Information
- Degree Disciplines:
- Podiatry
Notes
- Abstract:
- Sciatic nerve crush is followed by a short period of denervation which results in muscle fiber atrophy and changes in parameters, such as fiber size and myosin heavy chain (MHC) expression. Reinnervation interrupts and may sometimes reverse the pattern of atrophy and other altered parameters induced by the short period of denervation. The purpose of this study was to determine the changes in fiber size and muscle fiber proportion in the extensor digitorum longus (EDL) and soleus (SOL) following reinnervation. The left sciatic nerve of female Wistar rats was crushed for 20 seconds and the wound was closed with 6-0 nylon sutures. After 21 days, the EDL and SOL muscles were collected from each rat and frozen. Sections (10/tm) were cut for immunohistochemical staining for type 1, 2A, 2B, and 2X MHC isoforms, myosin ATPase staining, and H&E staining. The cross-sectional area (CSA) of each muscle fiber was measured using Scion Image Software 1.63 and the fiber type was determined by visual inspection. The differences in mean cross-sectional area (CSA) and mean fiber type proportions for reinnervated and control muscles were compared using an ANOVA test (Predictive Analytics Software [PASW] Statistics 17.0). In the fast-twitch EDL, there were significant changes in muscle fiber type proportions and evidence of transition towards a slower MHC profile throughout the muscle. Significant changes in fiber size but not proportion suggest that the SOL maintained its function as a slow — twitch muscle despite the atrophy of all fiber types observed 21 days after sciatic nerve crush.
- Thesis:
- Thesis (M.S.)--Barry University, 2009.
- Bibliography:
- Includes bibliographical references (leaves 51-57).
Record Information
- Source Institution:
- Barry University
- Holding Location:
- Barry University Archives and Special Collections
- Rights Management:
- Copyright Sumera Ackbarali. Permission granted to Barry University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
- Resource Identifier:
- QM471.A35 2009_AckbaraliSumera ( BU-Local )
- Classification:
- QM471.A35 2009 ( lcc )
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