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Thesis/Dissertation Information
- Degree Disciplines:
- Podiatry
Notes
- Abstract:
- This study is set out to examine the response of two different muscles to loss of innervations. Serial frozen sections (10 pm thick) of fast-twitch extensor digitorium longus muscles (EDL) and slow-twitch soleus muscles (SOL.) of the denervated and contralateral control hindlimbs at different time points (one, two and three weeks) were compared using different enzyme histochemical and immunohistochemical techniques. It was hypothesized that the fiber types in the two muscles would respond similarly to the loss of neuronal influence. Succinate dehydrogenase (SDH) was used to determine the oxidative capacity, while phosphorylase (PURL) was used to determine the glycolytic capacity. The expression of myosin heavy chain (Ml (MHC) isoforms were determined by immunohistochemistry. Based on the enzyme histochemical and immunohistochemical reactions, type 1 and 2A fibers in the denervated SOL showed severe progressive atrophy with time relative to the contralateral control SOL. Different fibers in the denervated EDL showed different extents of atrophy with time, type 2B fibers atrophied the most, then type 2X fibers and type 2A atrophied to a lesser extent relative to the contralateral control EDL. In addition, type 2B, 2X, and 2A fibers showed decreased glycolytic activities with time. In contrast, denervated type 1 fibers of the EDL maintained their size during the three week of denervation and expressed glycolytic activities. Thus, the results of the study showed that the fast-twitch EDL and slow-twitch SOL muscles responded differently to the loss innervation.
- Thesis:
- Thesis (M.S.)--Barry University, 2009.
- Bibliography:
- Includes bibliographical references (leaves 71-74).
Record Information
- Source Institution:
- Barry University
- Holding Location:
- Barry University Archives and Special Collections
- Rights Management:
- Copyright Kadhem Alkhenaizi. Permission granted to Barry University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
- Resource Identifier:
- SF762.A55 2009_AlkhenaiziKadhem ( BU-Local )
- Classification:
- SF762.A55 2009 ( lcc )
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